Why HUT ? Unlocking the protease riddle
I struggled to find a way to clear of the mucus congestion that plagued me for nearly four decades, I tried every remedy & potions under the sun to no avail, one day though I discovered digestive enzymes, I tried pork enzymes, but they lack intestinal enzymes as you can only buy pepsin from the stomach & pancreatin from the pancreas, but you cannot get intestin from the small intestines, so they are useless for digesting casein from dairy & gluten from cereals.
So I moved on to fruit enzymes, bromelain & papain with little success, these fruit enzymes only have a trypsin-like activity & since the human system produces 15 protease, one is next to useless, I have also found out since that papain is better supplied by eating fresh pawpaw.
The last enzymes, I tried are fungal enzymes, what a mine field, that is !
Enzymes usually have one function expressed in unit of activity/gm, so we have ALU/mg for lactase:
lactose + lactase = galactose + glucose, same with amylase DU/mg & lipase LU/mg.
With protease it is different, the human body produces 15 different protease,
1 from the stomach called pepsin,
5 from the pancreas called: trypsin, chymotrypsin, elastase, carboxypeptidase A & carboxypeptidase B &
9 small intestine protease: amino-protease, di-protease & tri-protease
All these protease have different activities, pepsin activity is not the same as elastase activity.
So why are all these different protease are expressed with only one accepted codex: HUT/mg?
What is HUT/mg?, it is the amount of the amino acid tyrosine released in hemoglobin when it is digested with a certain protease. Now if the assay (the activity) is done in an acid pH, it tells you that it has a pepsin activity or aspergillopepsin or aspartic protease as it is sometimes known.
If this assay is done in a neutral pH, it tells you that it is a chymotrypsin assay, a neutral protease.
So when you buy a product on the market with protease & HUT/mg, all you know is that it has pepsin or chymotrypsin or both, nothing else. What about the other 13 protease? I know it is impossible to list all the 15 protease & their unit of activity/mg, but you can try to list some crucial protease, which is why I decided to list 2 in my Zymax formulation.
The first one is DPPIV (E.C.18.104.22.168), this protease sits next to lactase in the small intestines & is usually deficient in people with Dairy casein intolerances. When DPPIV is deficient a normal by product of A1 casein called casomorphin-7 or BCM-7 :try-pro-phe-pro-gly-pro-ile starts to accumulate.
BCM is a potent protein fragment with opioid activity called exorphin which can only be digested by DPPIV.
The second protease is PEP (E.C.22.214.171.124), this protease is crucial for breaking exorphins derived from gluten, whereas Casein only produce BCM-7, gluten produces hundreds of exorphins, with size ranging from 5 to 33 amino acids. DPPIV can only digest exorphins once they have been broken down by PEP.
It is known that pure PEP can break down the longest undigested gluten fragment called mer33 into 6 peptides & 7 peptides which can be digested by brush border of the intestines including DPPIV.
Zymax contains 0.13 u/60 mg of DPPIV & 8.13 mu/37.5 mg of PEP
Philippe Thebault ND.Fellow ANTA